anti phospho her2 erbb2 (Cell Signaling Technology Inc)
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Anti Phospho Her2 Erbb2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+erbb2/Phospho-HER2%2FErbB2+(Tyr1221%2F1222)+Rabbit+mAb/pmc13041752-3-0-3
Average 96 stars, based on 311 article reviews
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Western Blot:Article Title: Recombinant Human Neuregulin1-β1 Significantly Reduces Schwannoma Growth in Mice. Article Snippet: A total of 4μm thick cross sections were used for hematoxylin and eosin (H&E) staining and immunohistochemical labeling of S100beta (S100b) (1:8000; Dako; GA50461-2), myelin protein zero (MPZ) (1:300; Bioss Antibodies; bs-0337R), p75 (1:200; Millipore; AB1554), and ErbB2 (1:500; Cell Signaling; 2,165) in an automated Ventana stainer (Ventana Medical Systems, USA), using standard antigen retrieval protocols (CC1st, no pretreatment for S100b-protein). .. Immunoblotting was completed as described in Morrison et al,19 using the following primary antibodies: c-Jun (1:1,000; Cell Signaling; 8,752), Article Title: Smoking aggravates neovascular age-related macular degeneration via Sema4D-PlexinB1 axis-mediated activation of pericytes. Article Snippet: .. The antibodies used for immunoblotting were as follow: anti-p-MLC (Cell Signaling Technology, #3671S,1:2000); anti-t-MLC (Cell Signaling Technology, #2672S, 1:2000); anti-α-SMA (Sigma, #A2547, 1:2000); anti-Collagen-I (Abcam, #ab34710,1:2000); anti-Collagen-I (Abcam, # ab270993,1:2000); anti-Flag (Sigma, #A8592-0.2MG, 1:4000); antiROR2 (Santa Cruze, #sc-374174, 1:500); anti-RhoA (Santa Cruze, #sc418, 1:1000); anti-c-MET (Cell Signaling Technology, #8198 T, 1:2000); Article Title: Smoking aggravates neovascular age-related macular degeneration via Sema4D-PlexinB1 axis-mediated activation of pericytes Article Snippet: .. The antibodies used for immunoblotting were as follow: anti-p-MLC (Cell Signaling Technology, #3671S,1:2000); anti-t-MLC (Cell Signaling Technology, #2672S, 1:2000); anti-α-SMA (Sigma, #A2547, 1:2000); anti-Collagen-I (Abcam, #ab34710,1:2000); anti-Collagen-I (Abcam, # ab270993,1:2000); anti-Flag (Sigma, #A8592-0.2MG, 1:4000); anti-ROR2 (Santa Cruze, #sc-374174, 1:500); anti-RhoA (Santa Cruze, #sc-418, 1:1000); anti-c-MET (Cell Signaling Technology, #8198 T, 1:2000); Article Title: Genomic and transcriptomic profiling of high-risk bladder cancer reveals diverse molecular and microenvironment ecosystems Article Snippet: Quantitative PCR was performed using Luna Universal qPCR master mix (NEB, M3003) and a BioRad CFX Connect Real Time System. .. Protein expression was verified using western blotting with these antibodies: Article Title: ECD co-operates with ERBB2 to promote tumorigenesis through upregulation of unfolded protein response and glycolysis. Article Snippet: Breast cancer cell lines, antibodies, cell lysate preparation and western blotting: SKBR3, BT474, HCC2218, UACC893, HCC1954 and HCC202 HER2+ breast cancer cell lines were purchased from ATCC (Manassas, VA) and were cultured in RPMI medium supplemented with 10% fetal bovine serum. .. The antibodies used were: Anti-ECD mouse monoclonal antibody (18); Anti-ECD (cat# 10192-1-AP, Proteintech); Immunohistochemistry:Article Title: GRB2 regulation of essential signaling pathways in the endometrium is critical for implantation and decidualization Article Snippet: .. For immunohistochemistry, dewaxed hydrated paraffin-embedded tissue sections were blocked with 10% normal goat serum in PBS (pH 7.5) and then incubated with primary antibody diluted in 10% normal goat serum in PBS (pH 7.5) overnight at 4 °C anti-GRB2 (1:2000 dilution; ab32037; Abcam), anti-PTGS2 (1:500 dilution; 160106; Cayman), anti-FOXO1 (1:500 dilution; CS-2880; Cell Signaling), anti-PGR (1:2000 dilution; CS-8757S; Cell Signaling), anti-EGFR (1:500 dilution; CS-2646; Cell Signaling), Article Title: ECD co-operates with ERBB2 to promote tumorigenesis through upregulation of unfolded protein response and glycolysis. Article Snippet: Breast cancer cell lines, antibodies, cell lysate preparation and western blotting: SKBR3, BT474, HCC2218, UACC893, HCC1954 and HCC202 HER2+ breast cancer cell lines were purchased from ATCC (Manassas, VA) and were cultured in RPMI medium supplemented with 10% fetal bovine serum. .. The antibodies used were: Anti-ECD mouse monoclonal antibody (18); Anti-ECD (cat# 10192-1-AP, Proteintech); Incubation:Article Title: GRB2 regulation of essential signaling pathways in the endometrium is critical for implantation and decidualization Article Snippet: .. For immunohistochemistry, dewaxed hydrated paraffin-embedded tissue sections were blocked with 10% normal goat serum in PBS (pH 7.5) and then incubated with primary antibody diluted in 10% normal goat serum in PBS (pH 7.5) overnight at 4 °C anti-GRB2 (1:2000 dilution; ab32037; Abcam), anti-PTGS2 (1:500 dilution; 160106; Cayman), anti-FOXO1 (1:500 dilution; CS-2880; Cell Signaling), anti-PGR (1:2000 dilution; CS-8757S; Cell Signaling), anti-EGFR (1:500 dilution; CS-2646; Cell Signaling), Membrane:Article Title: ERBB2 targeting reveals a significant suppression of tumorigenesis in murine endometrial cancer with Pten mutation Article Snippet: .. Membrane was blocked with Casein (0.5% v/v) prior to exposure to Expressing:Article Title: Genomic and transcriptomic profiling of high-risk bladder cancer reveals diverse molecular and microenvironment ecosystems Article Snippet: Quantitative PCR was performed using Luna Universal qPCR master mix (NEB, M3003) and a BioRad CFX Connect Real Time System. .. Protein expression was verified using western blotting with these antibodies: |
